 Michael BeenProfessor of Biochemistry
Contact InformationOffice Number: (919) 684-2858 e-mail been@biochem.duke.edu Lab Location214A Nanaline Duke Education
Research InterestsMechanism and structure of autocatalytic RNA. Interests in this lab are directed at understanding various aspects of RNA structure and function with a focus on catalytic RNAs. Most of the work currently is directed at developing a detailed understanding of the structure, dynamics and chemical mechanism of the self-cleaving RNAs sequences (ribozymes) in hepatitis delta virus (HDV). Lab MembersRecent Publications1. Abigail L. Brown, Anne T. Perrotta, Timothy S. Wadkins, and Michael D. Been The poly(A) site sequence in HDV RNA alters both extent and rate of self-cleavage of the antigenomic ribozyme. Nucleic Acids Research, 2008 More… 2. Klein, D.J, Been, M.D., and Ferré-D’Amaré, A.R. Essential Role of an Active-Site Guanine in glmS Ribozyme Catalysis. J. Am. Chem. Soc., 129, 14858 -14859, 2007. More… 3. Klein, D.J, Wilkinson, S.R., Been, M.D., and Ferré-D’Amaré, A.R. Requirement of helix P2.2 and nucleotide G1 for positioning of the cleavage site and cofactor in the glmS ribozyme. J.Mol.Biol. 373 178-189, 2007. More… 4. Perrotta, A.T. & Been, M.D.:A single nucleotide linked to a switch in metal ion reactivity preference in the HDV ribozymes. Biochemistry 46 5124-30, 2007. More… 5. Been, M.D.: HDV Ribozymes in “Hepatitis Delta Virus” , J.L. Casey, ed. Springer-Verlag Berlin Heidelberg. Current Topics in Microbiology and Immunology 307:47-65, 2006 More… 6. Perrotta, A.T., Wadkins, T.S. & Been, M.D.: Chemical rescue, multiple ionizable groups, and evidence for general-acid-base catalysis in the HDV genomic ribozyme. RNA 12:1282-1291, 2006 More… 7. Perrotta, A.T. & Been, M.D.:HDV ribozyme cleavage in monovalent cations. Biochemistry 45 11357-65. 2006. More… 8. Wilkinson, S.R, & Been, M.D.: A pseudoknot in the 3’ non-core region of the glmS ribozyme enhances self-cleavage activity. RNA 11:1788-1794, 2005. More… |
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